Champuru v1.0


Flot et al. (2006) Phase determination from direct sequencing of length-variable DNA regions Molecular Ecology Notes 6 (3), 627-630 link
Flot (2007) Champuru 1.0: a computer software for unraveling mixtures of two DNA sequences of unequal lengths Molecular Ecology Notes 7 (6), 974-977 link

If you would like to use Champuru offline, please find here a command-line version of Champuru as a perl script to run locally (you may have to modify the first line of the script depending on the location of perl on your system).

Mirror website: http://jfflot.mnhn.fr/champuru

Forward sequence: (Max = 1500 bp)

Reverse sequence: (Max = 1500 bp)

Reverse-complement reverse sequence



Champuru makes it possible to determine the haplotypes of heterozygous individuals without cloning, simply by analyzing the patterns of double peaks in the forward and reverse chromatograms. To get a quick feeling of how Champuru works, copy/paste ATSYKRMKY in the first field (forward chromatogram sequence) and WWKCTGAST in the second (reverse chromatogram sequence), then click 'Submit'.

And now, here is a 'real-life' example (original chromatograms: Forward and Reverse ).
Forward sequence: ATCGTGGGCGGCCGGCCCTGCCACAGACGGGGTTGTCACCCTCTGCGACGTGCCGTTCCAAGCAACTTAGGCAGGGCCCCTCCGCCTAGAAAGTGCTTCTCGCAACTACAACTCGCCGATGCAGGCATCGGAGATTTCAAATTTGAGCTCTTCCCGCTTCACTCGCCGTTACTGGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTAATATGCTTAAATTCAGCGGGTAGCCTTGCCTGATCTGAGGTCTGGAAGGCGATTCCTTTTTTCCTTTGAGATGCCGCCACCGCTACCCGGCGGCAGCAGAAAAAAGAATCGAATGGAGAAAGATTTGTTCCGTCAAAGCGATAGAGCCGTGGCCGTTTGGGGTACATTGTTCTATGATCCCCGCGCGACACCGGATGTCGCTTGGCGGATCTTTCTCCCTGAATTTCAAGGGACGCGGTAAACCGACCGGTCGGGCCGAGCAGCACCAGGGCTGGCTAGCTAGCGCACGACCGGTCATCTCGACCGCGACCCTCAACGCCGCACGAACCCGTTCACGGCGGGCGCGYSYCSSSSCCMYMYSCKMYASASRSGGRSMMSRSGSRSRCGCGCRCRCGSRKWYKCRCRMKRKRKRTKTKWRWAKASACWCWSASASASAYRYKCYYSKGRGARMMCMMRAGMGCSMYWTKYGYKYWMARAKWYKMKRWKWYWCWSWRWWYTCYGCWWYTCACWCTWYWTMTCRSMMTCKMKCTGA
Reverse sequence: GTGCRMTMTCAACACMCSASTCTCGMRACRCATMKYGKGSGSSSSSSSCYSYSMCASASRSGGKKKTSWCMCYCTSYGMSRYGYSSYKYYMMRMRMMWYWKRSRSRGSSCCYCYSCSYMKARARWGYKYYTCKCRMMWMYAMMWCKCSSMKRYRSRSRYMKSRGAKWTYWMAWWTKWGMKCTYYYCSCKYYWCWCKCSSYKWYWSKGGGRRWMYYYKTKWKWKTYTYTTYYYCYSCKYWTWWWWATRYKYWWAWWYWSMGSGKRKMSYYKYSYSWKMTSWGRKSTSKRRRRSGMKWYYYYTTTTYYYYTKWGAKRYSSCSMCMSCKMYMCSSSGSSRSMRSARAAAARARWMKMRWRKRGARARAKWTKTKYYSYSWMARMGMKAKAGMSSYGKSSSYKTKKGGKRYAYWKTKYTMTRWKMYCCSCGCGMSACMSSRKRTSKCKYKKSGSRKMTYTYTCYCYSWRWWTYWMRRGRSRCGSKRWAMMSMSMSSKSKSGSSSMGMRSMRCMMSRGSKSKSKMKMKMKMGCRCRMSMSSKSWYMTCKMSMSCGMSMCYCWMMRCSSCRCRMRMMCSYKYWCRSSGSGSGCGCGTCCCGGCCCCATCCGCTACAGACGGGGACCAGGCGGACGCGCGCACGCGGATTCGCACGATGGATGTTTGAATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTGCGTTCAAAGATTCGATGATTCACTGAAT

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